A WOMAN has died and two others have been injured after a car ran off a freeway and rolled north of Melbourne.

Ambulance Victoria paramedics arrived at the Hume Freeway, Wallan, just before 5am (AEST) today to find a Ford Laser hatch had overturned with three people inside.

They found the 53-year-old female front seat passenger dead and trapped in the wreckage, an ambulance spokesman said.

The driver, a 45-year-old woman from Moonee Ponds and her rear seat passenger, a 47-year-old Wallan woman, suffered serious injuries and were taken to the Royal Melbourne Hospital.

Meantime, a 36-year-old truck driver has died in hospital after he collided with another truck in Victoria's north.

Police believe the victim, from Cobram, was driving a small fruit truck when it hit the side of a B-double on the Goulburn Valley Highway, near Shepparton, just before 5pm (AEST) yesterday.

The fruit truck driver was flown to the Alfred Hospital in critical condition, but died in hospital on Thursday night.

The driver of the B-double was taken to The Goulburn Valley Base Hospital in a serious but stable condition.

The fatalities take the Victorian road toll to 100, four fewer road deaths than for the same time last year.

AAP