UP to 2000 homes in Victoria and NSW will be treated following the detection of fruit fly in Mildura.

An outbreak was declared in the area after a single fruit fly was caught in a trap in Cureton Avenue.

DPI senior market access officer, Gary D'Arcy, said as Mildura is a major fruit production area and lies within the Greater Sunraysia Pest Free Area, it is critical the pest be eradicated as soon as possible.

"Following the declaration, all host fruit will need to be certified under a DPI arrangement prior to leaving the 15km suspension zone and moving to other Victorian markets and sensitive interstate markets, including South Australia, Western Australia and the Murrumbidgee Irrigation Area of NSW," Mr D'Arcy said.

"An eradication program will commence within a 1.5 kilometre radius of the detection site on Cureton Avenue, which is located in Mildura."

Mr D'Arcy said the cooperation of residents will be crucial to the success of the program.

"We strongly encourage local residents to help by providing access to their backyards and by not removing any home-grown fresh fruit from their property," he said.

Most fruits, including citrus, stone and pome fruit, berries, grapes, tomatoes, capsicums and chillies are potential hosts of Queensland fruit fly.