NEW video of cruelty in Indonesian abattoirs shows 46 breaches of the supply chain checklist.

That's according to the RSPCA. The footage, shot by an unknown source in at least one Indonesian abattoir believed to be accredited under Australia's Exporter Supply Chain Assurance System and supplied to Animals Australia, was aired on national television last night.

The footage shows what are believed to be Australian animals being tripped over and falling onto their sides in Mark I restraint boxes - the same restraint boxes Australia's chief veterinary officer, Dr Andy Carroll, deemed unfit for use following an expose of animal cruelty in Indonesian abattoirs last year.

The footage also shows workers slitting the throats of cattle without stunning them first and workers cutting animals up while they were still alive.

RSPCA Australia's chief scientist Bidda Jones said the footage proved the ESCAS could not guarantee the humane treatment of Australian cattle shipped live to Indonesia.

"If it is the case, that the framework has been breached in Indonesia where so much attention has been focused and where all animals are individually traceable, what is the likelihood that standards will be upheld in other importing countries?'' Dr Bidda said.

"From what we've seen from this Indonesian footage, the RSPCA has significant concerns that the supply chain assurance system will not protect exported animals from cruelty.''

The RSPCA called on the government to enforce stunning before slaughter, a common practice in Australian abattoirs, for all live exports.

But the industry has hit out at claims the ESCAS was not improving animal welfare in Indonesian abattoirs supplied with Australian cattle.

"The new regulatory framework incorporates a process for the investigation and management of any instance of non-compliance. ESCAS holds individual exporters and supply chains responsible for animal welfare practices,'' a statement from the Australian Livestock Exporters Council said.

"Any activity that contravenes ESCAS will be investigated by the Australian Government and corrective action taken.''

Northern Territory Livestock Exporters Association chief executive Kevin Mulvahil said animal welfare remained a high priority for the live export industry and that Australian exporters were committed to ensuring good animal welfare outcomes in Indonesia.

"Of course, everyone is concerned about animal welfare and we take any animal welfare concerns very seriously,'' Mr Mulvahil said.

"Exporters are working really closely as an industry on the supply chain to make sure these issues do not occur again.''

The footage is being reviewed by the Department of Agriculture, Fisheries and Forestry and no further comment could be made until its origins were verified, he said.